gibson assembly cloning kit (New England Biolabs)
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gibson assembly cloning kit
Gibson Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+cloning+kit/Gibson+Assembly+Cloning+Kit/pmc13652249-465-15-19
Average 99 stars, based on 4143 article reviews
Gibson Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+cloning+kit/Gibson+Assembly+Cloning+Kit/pmc13652249-465-15-19
Average 99 stars, based on 4143 article reviews
gibson assembly cloning kit - by Bioz Stars,
2026-10
99/100 stars
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Plasmid Preparation:Article Title: Temporal loss of En1 during limb development causes distinct phenotypes Article Snippet: .. The SYN72 vector (pCMV-SynA [Addgene plasmid 174860]) was linearized by digestion with PacI (NEB), and the LSEE1&2 insert was integrated using the Article Title: Engineering an Optogenetic pH-Modulator in Bacteria. Article Snippet: .. Genes were inserted into the pAB50 plasmid backbone (Addgene) using PCR amplification followed by NEB Cloning:Article Title: Temporal loss of En1 during limb development causes distinct phenotypes Article Snippet: .. The SYN72 vector (pCMV-SynA [Addgene plasmid 174860]) was linearized by digestion with PacI (NEB), and the LSEE1&2 insert was integrated using the Article Title: Engineering an Optogenetic pH-Modulator in Bacteria. Article Snippet: .. Genes were inserted into the pAB50 plasmid backbone (Addgene) using PCR amplification followed by NEB Article Title: In Situ Genetic Engineering Facilitates MMP-9-Responsive TIMP1 Release Through Proteolytic Cleavage to Boost Diabetic Wound Healing. Article Snippet: .. Plasmids used in this study were constructed via Article Title: New insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo Article Snippet: .. To generate the AMH overexpression construct, emGFP-T2A-cAMH was cloned into the RCASBP(A) vector using the Article Title: Programmable histone acetylation enables reversible regulation of cellulase production in Myceliophthora thermophila. Article Snippet: PCR amplification was performed using Super-Fidelity DNA Polymerase (Vazyme, Nanjing, China). .. Plasmids were assembled using the Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the Polymerase Chain Reaction:Article Title: ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundles. Article Snippet: The organization of the cell’s cytoskeletal filaments is coordinated through a complex network of signaling cascades activated by both internal and external cues.. Two major actin regulatory pathways are signal transduction through Rho family GTPases and growth and proliferation signaling through the Hippo pathway.. These two pathways define the actin cytoskeleton, controlling foundational cellular attributes such as morphology, the organization of actin-based structures, and are hijacked to promote proliferation and motility in aggressive cancers. Article Title: Engineering an Optogenetic pH-Modulator in Bacteria. Article Snippet: .. Genes were inserted into the pAB50 plasmid backbone (Addgene) using PCR amplification followed by NEB Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the Gel Extraction:Article Title: ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundles. Article Snippet: The organization of the cell’s cytoskeletal filaments is coordinated through a complex network of signaling cascades activated by both internal and external cues.. Two major actin regulatory pathways are signal transduction through Rho family GTPases and growth and proliferation signaling through the Hippo pathway.. These two pathways define the actin cytoskeleton, controlling foundational cellular attributes such as morphology, the organization of actin-based structures, and are hijacked to promote proliferation and motility in aggressive cancers. Amplification:Article Title: Engineering an Optogenetic pH-Modulator in Bacteria. Article Snippet: .. Genes were inserted into the pAB50 plasmid backbone (Addgene) using PCR amplification followed by NEB Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the Transformation Assay:Article Title: Engineering an Optogenetic pH-Modulator in Bacteria. Article Snippet: .. Genes were inserted into the pAB50 plasmid backbone (Addgene) using PCR amplification followed by NEB Construct:Article Title: In Situ Genetic Engineering Facilitates MMP-9-Responsive TIMP1 Release Through Proteolytic Cleavage to Boost Diabetic Wound Healing. Article Snippet: .. Plasmids used in this study were constructed via Article Title: New insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo Article Snippet: .. To generate the AMH overexpression construct, emGFP-T2A-cAMH was cloned into the RCASBP(A) vector using the Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the Over Expression:Article Title: New insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo Article Snippet: .. To generate the AMH overexpression construct, emGFP-T2A-cAMH was cloned into the RCASBP(A) vector using the Clone Assay:Article Title: New insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo Article Snippet: .. To generate the AMH overexpression construct, emGFP-T2A-cAMH was cloned into the RCASBP(A) vector using the Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the other:Article Title: Triptans reprogram Schwann cells to drive medication-overuse headache via β-glycan/TGF-β3 signaling Article Snippet: From pCAG-G-FLAMP2 (#192782, RRID:Addgene_192782, Addgene), G-FLAMP2 was cloned (EcoRI/XbaI) into pEG backbone (P33/P34). Stable Transfection:Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the Expressing:Article Title: Somatic mutations in CSK disrupt immune regulation in a Chinese aplastic anemia cohort. Article Snippet: .. To construct cells stably over-expressing CSK, full-length wild-type CSK cDNA was amplified from one patient DNA by PCR and cloned into a lentiviral expression vector (pLenti-CMV-GFP-Puro; Addgene, #17448) using the |